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HLA-E*01:03 (Tetramer) protein (Biotin,His-Avi Tag)

宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7454270
发货至: 中国
  • 抗原
    HLA-E*01:03
    蛋白类型
    Recombinant
    产品特性
    Tetramer
    宿主
    资源
    • 3
    HEK-293 Cells
    标记
    Biotin,His-Avi Tag
    序列
    Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M)and VMAPRTLVL peptide
    产品特性
    Recombinant Biotinylated Human HLA-E*01:03 Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    4 °C,-80 °C
    储存方法
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 抗原
    HLA-E*01:03
    背景
    HLAE, sHLA-E, HLAE, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, MHC, QA1. HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with ≤2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
    分子量
    258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
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