HLA-A*24:02 (Monomer) protein (GP100 Intron 4)
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- 抗原
- HLA-A*24:02
- 蛋白类型
- Recombinant
- 产品特性
- Monomer
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- GP100 Intron 4
- 原理
- Human HLA-A*24:02 & B2M & GP100 Intron 4 (VYFFLPDHL) Monomer Protein
- 序列
- Gly25-Thr305 (HLA-A*24:02), Ile21-Met119 (B2M) and VYFFLPDHL peptide
- 特异性
- Uni-Prot: AAA59600.1 (HLA-A*24:02), P61769 (B2M), VYFFLPDHL
- 产品特性
- Recombinant Human HLA-A*24:02 & B2M & GP100 Intron 4 (VYFFLPDHL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*24:02), Ile21-Met119(B2M) and VYFFLPDHL peptide.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- HLA-A*24:02
- 背景
- Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
- 分子量
- 50.40 kDa. Due to glycosylation, the protein migrates to 55-63 kDa based on Tris-Bis PAGE result.
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