IL12 Protein (AA 23-328) (His tag)
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- 抗原 See all IL12 蛋白
- IL12 (Interleukin 12 (IL12))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-328
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宿主
- Cynomolgus
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资源
- HEK-293 Cells
- 标记
- This IL12 protein is labelled with His tag.
- 原理
- Cynomolgus IL-12 Protein
- 序列
- Ile23-Ser328 (IL-12B) & Arg57-Ser253 (IL-12A)
- 特异性
- Uni-Prot: G7P6S2 (IL-12B), XP_005546300.2 (IL-12A)
- 产品特性
- Recombinant Cynomolgus IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328 (IL-12B) & Arg57-Ser253 (IL-12A).
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product IL12 蛋白
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- IL12 (Interleukin 12 (IL12))
- 别名
- IL-12 (IL12 产品)
- 别名
- CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein
- 背景
- Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
- 分子量
- 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on Tris-Bis PAGE result.
- UniProt
- G7P6S2
- 途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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