HLAG
(HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
蛋白类型
Recombinant
产品特性
Monomer
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This HLAG protein is labelled with Biotin,HLA-G.
原理
Biotinylated Cynomolgus HLA-G & B2M & Peptide (RIIPRHLQL) Monomer Protein
序列
Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide
特异性
Uni-Prot: E0WKX9 (HLA-G), Q8SPW0 (B2M), RIIPRHLQL
产品特性
Recombinant Biotinylated Cynomolgus HLA-G & B2M & Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
The affinity constant of 0.23 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
HLAG
(HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
MHC-G Protein, B-F Protein, B-F-S04 Protein, B-F-S05 Protein, B-F-S06 Protein, B-F-S07 Protein, B-FI Protein, B-FIV Protein, BF2 Protein, BFa2 Protein, BFw-03 Protein, BFw-05 Protein, BFz-01 Protein, major histocompatibility complex, class I, G Protein, MHC BF1 class I Protein, HLA-G Protein, BF1 Protein
背景
HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
分子量
50.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.