This FGL2 protein is labelled with Biotin,His-Avi Tag,DYKDDDDK Tag.
原理
Biotinylated Human FGL2 Protein
序列
Val205-Pro439
产品特性
Recombinant Biotinylated Human FGL2 Protein is expressed from HEK293 with His-Avi tag and Flag tag at the N-Terminus.It contains Val205-Pro439.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-FGL2 Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGL2, His Tag with the EC50 of 0.34μg/ml determined by ELISA. See testing image for detail.
FGL2
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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背景
Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.
分子量
31.2 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.