Glypican 3 Protein-VLP (GPC3) (AA 510-554)
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- 抗原 See all Glypican 3 (GPC3) 蛋白
- Glypican 3 (GPC3)
- 蛋白类型
- VLP
- 生物活性
- Active
- 产品特性
- AA 510-554
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Immunogen (Imm), ELISA, Functional Studies (Func), Surface Plasmon Resonance (SPR)
- 原理
- Human GPC3 Protein-VLP
- 序列
- Gly510-Asn554
- 产品特性
- Recombinant Human GPC3 Protein-VLP is expressed from E.coli.It contains Gly510-Asn554.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GPC3 VLP at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 20.2ng/ml determined by ELISA.
- Top Product
- Discover our top product GPC3 蛋白
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Glypican 3 (GPC3) (AA 25-559) protein (His tag)
GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glypican 3 (GPC3) (AA 25-559) protein (His tag)GPC3 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glypican 3 (GPC3) (AA 25-559) protein (His-Avi Tag,Biotin)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glypican 3 (GPC3) protein (Fc Tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Glypican 3 (GPC3) (AA 438-554) (Active) protein-VLPGPC3 宿主: 人 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC Imm, ELISA, Func, SPR Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (Fc Tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE Func Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag,AVI tag,Biotin)GPC3 宿主: 人 宿主: HEK-293 Cells Recombinant >85 % as determined by SDS-PAGE. Active
Glypican 3 (GPC3) protein (His tag)GPC3 宿主: 小鼠 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
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- 应用备注
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- Antibody Discovery: Immunization, Screening, Functional Characterization
- Affinity determination: ELISA, SPR
- In vivo pharmacokinetic analysis
- CMC method development
- CAR-T Positive Rate Detection
- Blood sample determination: ELISA
- 说明
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Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.
Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.
Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Supplied as 0.22μm filtered solution in 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 0.1 % Tween20 ( pH 7.7).
- 储存条件
- -80 °C
- 储存方法
- Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Glypican 3 (GPC3)
- 别名
- GPC3 (GPC3 产品)
- 别名
- GPC3 Protein, sgb Protein, dgsx Protein, sdys Protein, sgbs Protein, oci-5 Protein, sgbs1 Protein, DGSX Protein, GTR2-2 Protein, MXR7 Protein, OCI-5 Protein, SDYS Protein, SGB Protein, SGBS Protein, SGBS1 Protein, Glypican-3 Protein, glypican 3 Protein, gpc3 Protein, GPC3 Protein, Gpc3 Protein
- 背景
- Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
- 分子量
- 14 kDa.
- 途径
- Glycosaminoglycan Metabolic Process
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