TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This TNFRSF10A protein is labelled with His-Avi Tag,Biotin.
序列
Pro34-Asn239
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TRAIL, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31μg/ml determined by ELISA. The affinity constant of 0.315 nM as determined in SPR assay (Biacore T200). See testing image for detail.
TNFRSF10A
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 92 % as determined by reducing SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
APO2 Protein, CD261 Protein, DR4 Protein, TRAILR-1 Protein, TRAILR1 Protein, TNFRSF10A Protein, TNF receptor superfamily member 10a Protein, tumor necrosis factor receptor superfamily member 26 Protein, tumor necrosis factor receptor superfamily, member 10a Protein, TNFRSF10A Protein, LOC510798 Protein
背景
DR4, CD261, TNFRSF10A, TRAIL-R, APO2, TRAIL R1, MGC9365,Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily.The expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells.
分子量
24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.