TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
蛋白类型
Recombinant
产品特性
AA 30-211
宿主
人
资源
HEK-293 Cells
标记
This TNFRSF1A protein is labelled with His tag.
原理
Human TNFR1/CD120a/TNFRSF1A Protein
序列
Leu30-Thr211
产品特性
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu30-Thr211.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TNFR1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 4.8ng/ml determined by ELISA. See testing image for detail.
TNFRSF1A
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 96 % as determined by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF1A
(Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.
分子量
23.3 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.