TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
蛋白类型
Recombinant
产品特性
AA 23-257
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This TNFRSF1B protein is labelled with His tag.
原理
Cynomolgus TNFR2/CD120b/TNFR1B Protein
序列
Leu23-Asp257
产品特性
Recombinant Cynomolgus TNFR2/CD120b/TNFR1B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asp257.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus TNFR2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.
TNFRSF1B
宿主: 人
宿主: 小麦胚
Recombinant
WB, ELISA, AP, AA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
分子量
26.1 kDa. Due to glycosylation, the protein migrates to 49-60 kDa based on Tris-Bis PAGE result.