TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
蛋白类型
Recombinant
产品特性
AA 26-143
宿主
小鼠
资源
HEK-293 Cells
标记
This TIGIT protein is labelled with Fc Tag.
原理
Mouse TIGIT Protein
序列
Gly26-Thr143
产品特性
Recombinant Mouse TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly26-Thr143.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse CD155, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail.
TIGIT
宿主: Cynomolgus
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein
背景
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
分子量
39.7 kDa. Due to glycosylation, the protein migrates to 48-57 kDa based on Tris-Bis PAGE result.