Immobilized Human Mature TGF beta 1, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA. See testing image for detail.
TGFB1
宿主: 小鼠
宿主: 人细胞
Recombinant
> 95 % as determined by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in 50 mM Glycine, 150 mM NaCl ( pH 2.5).
缓冲液
Lyophilized from 0.22μm filtered solution in 50 mM Glycine, 150 mM NaCl ( pH 2.5). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CEDLAP, DPD1, TGF beta1, TGFB, TGFB1, TGFbeta, TGF-beta-1,TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.
分子量
13.2 kDa. Due to glycosylation, the protein migrates to 14-15 kDa based on Tris-Bis PAGE result.