Recombinant Cynomolgus SOST/Sclerostin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln69-Tyr258.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus SOST, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
分子量
22.58 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.