Recombinant Human CD48/SLAMF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Ser220.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD48, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.45μg/ml determined by ELISA. See testing image for detail.
CD48
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 85 % as determined by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.
分子量
23.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.