Recombinant Mouse R spondin 1/RSPO1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Gln265.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse R spondin 1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA. See testing image for detail.
RSPO1
宿主: 人
宿主: 人细胞
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Liquid
缓冲液
Supplied as 0.22μm filtered solution in 20 mM MES, 150 mM NaCl, 200 mM Arginine ( pH 5.5).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
分子量
28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.