Recombinant Cynomolgus PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Pro21-Gln167.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus PD-1, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA. The affinity constant of 1.28 μM as determined in SPR assay (Biacore T200). See testing image for detail.
PDCD1
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
分子量
42.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.