This PLAUR protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Mouse uPAR/PLAUR Protein
序列
Leu24-Gly298
产品特性
Recombinant Biotinylated Mouse uPAR/PLAUR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gly298.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human PLAU, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse uPAR isoform 1, His Tag with the EC50 of 2.57μg/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
分子量
32.92 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.