KLRC1
(Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
标记
This KLRC1 protein is labelled with His-Avi Tag.
序列
Arg100-Leu233
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human NKG2A, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 0.79μg/ml determined by ELISA. The affinity constant of 0.23μM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
KLRC1
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
有效期
12 months
抗原
KLRC1
(Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))
rNKG2A Protein, NKG2-A Protein, NKG2A Protein, KLRC1 Protein, CD159A Protein, NKG2 Protein, CD159a Protein, NKG2B Protein, killer cell lectin like receptor C1 Protein, NKG2-A/NKG2-B type II integral membrane protein-like Protein, killer cell lectin-like receptor subfamily C, member 3 Protein, killer cell lectin-like receptor subfamily C, member 1 Protein, Klrc1 Protein, KLRC1 Protein, NKG2A Protein, KLRC3 Protein
背景
NKG2A, NKG2-A, CD94, KLRD1, KP43, KLRC1, CD159A,NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 Molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells.
分子量
18.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.