MICA
(MHC Class I Polypeptide-Related Sequence A (MICA))
蛋白类型
Recombinant
产品特性
AA 1-284
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This MICA protein is labelled with His tag.
原理
Cynomolgus MICA Protein
序列
Glu1-Trp284
产品特性
Recombinant Cynomolgus MICA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu1-Trp284.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus MICA, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.55μg/ml determined by ELISA. See testing image for detail.
MICA
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
MICA
(MHC Class I Polypeptide-Related Sequence A (MICA))
MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein
背景
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
分子量
33.53 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.