MAG Protein (AA 20-516) (His tag)
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- 抗原 See all MAG 蛋白
- MAG (Myelin Associated Glycoprotein (MAG))
- 蛋白类型
- Recombinant
- 产品特性
- AA 20-516
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This MAG protein is labelled with His tag.
- 原理
- Human Siglec-4a/MAG Protein
- 序列
- Gly20-Pro516
- 产品特性
- Recombinant Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Pro516.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Siglec-4a, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS, 100 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- MAG (Myelin Associated Glycoprotein (MAG))
- 别名
- Siglec-4a (MAG 产品)
- 别名
- GMA Protein, S-MAG Protein, SIGLEC-4A Protein, SIGLEC4A Protein, siglec-4 Protein, MAG Protein, Gma Protein, siglec-4a Protein, myelin associated glycoprotein Protein, myelin-associated glycoprotein Protein, MAG Protein, mag Protein, Mag Protein
- 背景
- Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
- 分子量
- 55.8 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
- UniProt
- P20916
- 途径
- Neurotrophin Signaling Pathway
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