LRP6
(Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
蛋白类型
Recombinant
产品特性
AA 20-630
宿主
人
资源
HEK-293 Cells
标记
This LRP6 protein is labelled with mFc Tag.
原理
Human LRP-6 Protein
序列
Ala20-Pro630
产品特性
Recombinant Human LRP-6 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Pro630.
纯度
> 90 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human DKK1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 2.1μg/ml determined by ELISA. See testing image for detail.
LRP6
宿主: 人
宿主: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
LRP6
(Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
lrp6-A Protein, Xlrp6 Protein, adcad2 Protein, lrp6 Protein, LRP6 Protein, ADCAD2 Protein, C030016K15Rik Protein, Cd Protein, Gw Protein, LDL receptor related protein 6 Protein, LDL receptor related protein 6 L homeolog Protein, LDL receptor related protein 6 S homeolog Protein, low density lipoprotein receptor-related protein 6 Protein, low-density lipoprotein receptor-related protein 6 Protein, LRP6 Protein, lrp6.L Protein, lrp6.S Protein, lrp6 Protein, Lrp6 Protein, LOC590395 Protein
背景
Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.
分子量
95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.