Lrig1
(Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))
蛋白类型
Recombinant
产品特性
AA 35-779
宿主
人
资源
HEK-293 Cells
标记
This Lrig1 protein is labelled with His tag.
原理
Human LRIG1 Protein
序列
Ala35-Ser779
产品特性
Recombinant Human LRIG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala35-Ser779.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LRIG1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-LRIG1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.
Lrig1
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl, 1 mM EDTA ( pH 5.0).
缓冲液
Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl, 1 mM EDTA ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.
储存液
Other preservative
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
Lrig1
(Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))
LIG-1 Protein, LIG1 Protein, D6Bwg0781e Protein, Img Protein, leucine rich repeats and immunoglobulin like domains 1 Protein, leucine-rich repeats and immunoglobulin-like domains 1 Protein, LRIG1 Protein, Lrig1 Protein
背景
The leucine-rich repeats and immunoglobulin-like domains (LRIG)-1 is a tumor suppressor gene that belongs to the LRIG family. LRIG1 expression has prognostic significance in various human cancers. Somatic mutations, which are associated with a certain rate of response to targeted therapies, are ubiquitously found in human non-small cell lung cancer (NSCLC). LRIG1 was an independent prognostic factor for OS of NSCLC patients. LRIG1 in combination with other clinicopathological risk factors was a stronger prognostic model than clinicopathological risk factors alone.
分子量
83 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Tris-Bis PAGE result.