This LILRB3 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human LILRB3/CD85a/ILT5 Protein
序列
Gly24-Glu443
产品特性
Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human LILRB3, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD85A Protein, HL9 Protein, ILT-5 Protein, ILT5 Protein, LILRA6 Protein, LIR-3 Protein, LIR3 Protein, PIRB Protein, Gp91 Protein, Lilrb3 Protein, PIR-B Protein, Pirb Protein, NILR-1 Protein, LILRB3 Protein, leukocyte immunoglobulin like receptor B3 Protein, paired Ig-like receptor B Protein, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 Protein, LILRB3 Protein, Pirb Protein
背景
Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.
分子量
50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Tris-Bis PAGE result.