LILRB4
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))
蛋白类型
Recombinant
产品特性
AA 22-256
宿主
人
资源
HEK-293 Cells
标记
This LILRB4 protein is labelled with Fc Tag.
原理
Human LILRB4/CD85k/ILT3 Protein
序列
Gln22-Arg256
产品特性
Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Arg256.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LILRB4, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. The affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Crystallography grade
LILRB4
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
LILRB4
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))
CD85K Protein, ILT-3 Protein, ILT3 Protein, LIR-5 Protein, LIR5 Protein, Gp49b Protein, HM18 Protein, gp49 Protein, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4 Protein, leukocyte immunoglobulin like receptor B4 Protein, leukocyte immunoglobulin-like receptor, subfamily B, member 4A Protein, LILRB4 Protein, Lilrb4a Protein
背景
LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
分子量
52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.