Recombinant Human Integrin alpha V beta 3 (ITGAV & ITGB3) Heterodimer Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human ITGAV&ITGB3, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD51 Protein, MSK8 Protein, VNRA Protein, VTNR Protein, integrin subunit alpha V Protein, ITGAV Protein
背景
ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.
分子量
131.90 kDa (ITGAV)&101.79 kDa (ITGB3). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAV)&105-130 kDa (ITGB3) based on Tris-Bis PAGE result.