IL1RAP
(Interleukin 1 Receptor Accessory Protein (IL1RAP))
蛋白类型
Recombinant
产品特性
AA 21-359
宿主
人
资源
HEK-293 Cells
标记
This IL1RAP protein is labelled with Fc Tag.
原理
Human IL-1R3/IL-1 RAcP Protein
序列
Ser21-Glu359
产品特性
Recombinant Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser21-Glu359.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-1R3, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
IL1RAP
(Interleukin 1 Receptor Accessory Protein (IL1RAP))
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
分子量
65.8 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.