This IL21 Receptor protein is labelled with His tag.
原理
Human IL-21R Protein
序列
Cys20-Glu232
产品特性
Recombinant Human IL-21R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys20-Glu232.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-21 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, His Tag with the EC50 of 24.3ng/ml determined by ELISA. See testing image for detail.
IL21R
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
IL-21 and IL-21R were highly expressed in the lesional skin and peripheral blood of psoriasis patients. IL-21 promoted CD4 T cells proliferation and Th17 cells differentiation and inhibiting Treg cells differentiation by upregulating RORγt expression and downregulating Foxp3 expression, with increased expression and secretion of IL-17A and IL-22.Microbial translocation and the associated immune activation during HIV-1 infection may lead to high expression levels of the IL-21R activation marker in RM B cells, a feature associated with increased apoptosis and a reduced number of these cells in the circulation.
分子量
25.65 kDa. Due to glycosylation, the protein migrates to 43-50 kDa based on Tris-Bis PAGE result.