This IL4 Receptor protein is labelled with His tag.
原理
Cynomolgus IL-4 R alpha/CD124 Protein
序列
Met26-Arg232
产品特性
Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met26-Arg232.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-4, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 36.5ng/ml determined by ELISA. The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.
IL4R
宿主: 大鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by SDS-PAGE
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family.The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.
分子量
24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.