This IL18BP protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human IL-18BP Protein
序列
Thr31-Gly194
产品特性
Recombinant Biotinylated Human IL-18BP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr31-Gly194.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-18 at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-18BP, His Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.
IL18BP
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 95 % by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
IL18BP Protein, IL-18BP Protein, Igifbp Protein, MC54L Protein, IL18BPa Protein, interleukin 18 binding protein Protein, IL18BP Protein, Il18bp Protein
背景
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
分子量
20.5 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Tris-Bis PAGE result.