IL18RAP
(Interleukin 18 Receptor Accessory Protein (IL18RAP))
蛋白类型
Recombinant
产品特性
AA 20-356
宿主
人
资源
HEK-293 Cells
标记
This IL18RAP protein is labelled with Fc Tag.
原理
Human IL-18RAP Protein
序列
Phe20-Arg356
产品特性
Recombinant Human IL-18RAP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe20-Arg356.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-18RAP, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
IL18RAP
(Interleukin 18 Receptor Accessory Protein (IL18RAP))
IL18RAP Protein, ACPL Protein, CD218b Protein, CDw218b Protein, IL18RB Protein, interleukin 18 receptor accessory protein Protein, IL18RAP Protein, Il18rap Protein
背景
Lactoferrin (LTF), peptidoglycan recognition protein 1 and IL-18 receptor accessory protein (IL18RAP) remained significantly elevated in peripheral monocytes of hypertensive individuals in a separate validation cohort. Interestingly, IL18RAP expression associated with MAP in a cohort of African Americans..
分子量
65.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.