> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 0.05 EU per μg by the LAL method
Biological Activity Comment
Measured in a cell proliferation assay using CTLL2 cells. The ED50 for this effect is 0.1 0.3 ng/mL. The specific activity of recombinant human IL15 is > 1 x 10^7 units/mg, which is calibrated against the human IL15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NACI (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freezethaw cycles.
L15, IL-15, IL-15MGC9721, Interleukin-15,Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases.IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.