Immobilized Human IL-4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.
IL4
宿主: 人
宿主: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
IL4, IL-4, interleukin 4, MGC79402, pitrakinra, B cell growth factor 1, BCDF, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, BSF-1,Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. .
分子量
17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.