This ErbB2/Her2 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human Her2/ErbB2 Domain 4 Protein
序列
Pro489-Cys630
产品特性
Recombinant Biotinylated Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro489-Cys630.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
ErbB2/Her2
宿主: 人
宿主: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.
分子量
18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.