This ERBB3 protein is labelled with His-Avi Tag,Biotin.
序列
Ser20-Thr643
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human Her3 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 17.6ng/ml determined by ELISA. See testing image for detail.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
ErbB3, ErbB-3, HER3, HER3c-erbB-3, LCCS2, MDA-BF-1, MGC88033, p180-ErbB3, p45-sErbB3, p85-sErbB3, ERBB3,Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).ation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
分子量
71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.