TNFRSF18
(Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))
蛋白类型
Recombinant
产品特性
AA 26-161
宿主
人
资源
HEK-293 Cells
标记
This TNFRSF18 protein is labelled with Fc Tag.
原理
Human GITR/TNFRSF18 Protein
序列
Gln26-Glu161
产品特性
Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln26-Glu161.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human GITR Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
TNFRSF18
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF18
(Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))
GITR Protein, TNFRSF18 Protein, AITR Protein, Gitr Protein, CD357 Protein, GITR-D Protein, TNF receptor superfamily member 18 Protein, tumor necrosis factor receptor superfamily, member 18 Protein, TNFRSF18 Protein, Tnfrsf18 Protein
背景
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
分子量
41.3 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.