Recombinant Human GPVI Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Lys267.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human GPVI, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
BDPLT11 Protein, GPIV Protein, GPVI Protein, 9830166G18Rik Protein, Gm469 Protein, Gpvi Protein, GP6 Protein, glycoprotein VI platelet Protein, glycoprotein 6 (platelet) Protein, glycoprotein VI Protein, platelet glycoprotein VI Protein, GP6 Protein, Gp6 Protein, LOC103346235 Protein
背景
Although platelets are best known for their role in hemostasis, they are also crucial in development, host defense, inflammation, and tissue repair. Many of these roles are regulated by the immune-like receptors glycoprotein VI (GPVI) and C-type lectin receptor 2 (CLEC-2), which signal through an immunoreceptor tyrosine-based activation motif (ITAM). GPVI is activated by collagen in the subendothelial matrix, by fibrin and fibrinogen in the thrombus, and by a remarkable number of other ligands.
分子量
28 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.