This GFRAL protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human GFRAL/GFR alpha-like Protein
序列
Ser19-Glu351
产品特性
Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human GDF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 0.1μg/ml determined by ELISA. See testing image for detail.
Crystallography grade
GFRAL
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, ELISA, SDS, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
RGD1565220 Protein, C6orf144 Protein, GRAL Protein, UNQ9356 Protein, bA360D14.1 Protein, AY457637 Protein, Gral Protein, GDNF family receptor alpha like Protein, Gfral Protein, GFRAL Protein
背景
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
分子量
40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.