Recombinant Cynomolgus FOLR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln9-His187.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus FOLR2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA. See testing image for detail.
FOLR2
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in 50 mM Tris,100 mM Glycine ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
FOLR1 and FOLR2 are equipped with cellular glycosylphosphatidylinositol (GPI) anchors. FOLR1 is secreted from epithelia with or without a micelle-encapsulated GPI-anchor into milk and other body fluids/secretions, e.g. semen where its interaction with spermatozoa indicates a role in male fertility. FOLR1 and FOLR2 serve as serum biomarkers of various diseases. FOLR3 possesses no GPI-anchor and originates from secretory granules of neutrophil granulocytes, its concentration in serum correlates to the FOLR3 content in leukocytes and rises with increased leukocyte counts (infection, malignancy and pregnancy).
分子量
25.5 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.