EGF 蛋白
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- 抗原 See all EGF 蛋白
- EGF (Epidermal Growth Factor (EGF))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 序列
- Asn971-Arg1023
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human EGF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA. The affinity constant of 0.05 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Epidermal Growth Factor (EGF) (Active) protein
EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) proteinEGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (AA 977-1023) protein (GST tag)EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- EGF (Epidermal Growth Factor (EGF))
- 别名
- EGF (EGF 产品)
- 背景
- HOMG4, EGF, URG,The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
- 分子量
- 5.6 kDa same as Tris-Bis PAGE result.
- 途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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