This Desmoglein 2 protein is labelled with His tag.
原理
Human DSG-2/Desmoglein-2 Protein
序列
Ala50-Gly609
产品特性
Recombinant Human DSG-2/Desmoglein-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala50-Gly609.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human DSG-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DSG-2 Antibody, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Desmoglein-2 (DSG-2) as the primary high-affinity receptor used by adenoviruses Ad3, Ad7, Ad11 and Ad14. These serotypes represent key human pathogens causing respiratory and urinary tract infections. In epithelial cells, adenovirus binding of DSG-2 triggers events reminiscent of epithelial-to-mesenchymal transition, leading to transient opening of intercellular junctions.
分子量
63.6 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.