TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
蛋白类型
Recombinant
产品特性
AA 56-182
宿主
人
资源
HEK-293 Cells
标记
This TNFRSF10B protein is labelled with Fc Tag.
原理
Human TRAIL R2/DR5/TNFRSF10B Protein
序列
Ile56-Glu182
产品特性
Recombinant Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile56-Glu182.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TRAIL at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 20ng/ml determined by ELISA. See testing image for detail.
TNFRSF10B
宿主: 人
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
TNFRSF10B
宿主: 人
宿主: 小麦胚
Recombinant
WB, ELISA, AP, AA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in 50 mM Tris, 100 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFRSF10B
(Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))
DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
分子量
41.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.