This DKK1 protein is labelled with Fc-Avi Tag,Biotin.
原理
Biotinylated Human DKK1 C terminal Domain Protein
序列
Met178-His266
产品特性
Recombinant Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met178-His266.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LRP-6, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA. See testing image for detail.
DKK1
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.0).
缓冲液
Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
分子量
38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.