This DKK1 protein is labelled with Fc-Avi Tag,Biotin.
原理
Biotinylated Human DKK1 N terminal Domain Protein
序列
Thr32-Asp142
产品特性
Recombinant Biotinylated Human DKK1 N terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Thr32-Asp142.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
DKK1
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
分子量
40.25 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.