This PF4 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human CXCL4 Protein
序列
Glu32-Ser101
产品特性
Recombinant Biotinylated Human CXCL4 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Glu32-Ser101.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CXCL4 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 2.3μg/ml determined by ELISA. See testing image for detail.
PF4
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
>95 % as determined by SDS-PAGE
Func
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl ( pH 3.0).
缓冲液
Lyophilized from 0.22μm filtered solution in 4 mM HCl ( pH 3.0). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.
分子量
10.68 kDa. The protein migrates to 13 kDa based on Tris-Bis PAGE result.