This CXCL13 protein is labelled with His-SUMO Tag.
原理
Human CXCL13/BCA-1 Protein
序列
Val23-Pro109
产品特性
Recombinant Human CXCL13/BCA-1 Protein is expressed from E.coli with His tag and Sumo tag at the N-Terminus.It contains Val23-Pro109.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CXCL13 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CXCL13, His Tag with the EC50 of 0.54μg/ml determined by ELISA. See testing image for detail.
Supplied as 0.22μm filtered solution in 0.1M Glycine, 0.3M NaCl, 0.2M L-arginine, 10 % Glycerol ( pH 3.0).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice.
分子量
22.9 kDa. The protein migrates to 26-28 kDa based on Tris-Bis PAGE result.