This CSF1R protein is labelled with His-Avi Tag,Biotin.
序列
Ile20-Glu512
纯度
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CSF R Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human M-CSF R, His Tag with the EC50 of 45.4ng/ml determined by ELISA. The affinity constant of 0.81 μM as determined in SPR assay (Biacore T200). See testing image for detail.
CSF1R
宿主: 人
宿主: Baculovirus infected Insect Cells
Recombinant
> 82 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
储存条件
4 °C,-80 °C
储存方法
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD115, CSF1R, CSF-1-R, CSFR, FMSFIM2, M-CSF R, MCSFR, M-CSFR, C-FMS, FIM2, FMS, HDLS,CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
分子量
57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.