This SARS-CoV-2 Spike protein is labelled with His tag.
原理
SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein
序列
Arg319-Phe541 (N501Y,K417N,E484K)
产品特性
Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y,K417N,E484K).
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.3ng/ml determined by ELISA. See testing image for detail.
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
物质类
Viral Protein
背景
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
分子量
26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.