Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met113-Leu261.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD40, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 0.13μg/ml determined by ELISA. See testing image for detail.
CD40LG
宿主: 小鼠
宿主: 大肠杆菌(E. Coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. .
分子量
74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.