CD3 epsilon Protein (CD3E) (Fc Tag)
-
- 抗原 See all CD3 epsilon (CD3E) 蛋白
- CD3 epsilon (CD3E)
- 蛋白类型
- Recombinant
-
宿主
- Cynomolgus
-
资源
- HEK-293 Cells
- 标记
- This CD3 epsilon protein is labelled with Fc Tag.
- 序列
- Gln22-Asp117
- 纯度
- > 95% as determined by Tris-Bis PAGE
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
-
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 别名
- cd3e Protein, CD3E Protein, t3e Protein, tcre Protein, AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, T3E Protein, TCRE Protein, CD3e molecule Protein, CD3 antigen, epsilon polypeptide Protein, cd3e Protein, CD3E Protein, Cd3e Protein
- 背景
- CD3e, CD3E, T3E, FLJ18683, TCRE, CD3-epsilon, IMD18,CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
- 分子量
- 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
-