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CD3 epsilon Protein (CD3E) (Fc Tag)

CD3E 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
产品编号 ABIN7274301
发货至: 中国
  • 抗原 See all CD3 epsilon (CD3E) 蛋白
    CD3 epsilon (CD3E)
    蛋白类型
    Recombinant
    宿主
    • 22
    • 9
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cynomolgus
    资源
    • 20
    • 8
    • 5
    • 5
    • 2
    • 1
    HEK-293 Cells
    标记
    This CD3 epsilon protein is labelled with Fc Tag.
    序列
    Gln22-Asp117
    纯度
    > 95% as determined by Tris-Bis PAGE
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
    储存条件
    4 °C,-80 °C
    储存方法
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    CD3 epsilon (CD3E)
    别名
    CD3E (CD3E 产品)
    背景
    CD3e, CD3E, T3E, FLJ18683, TCRE, CD3-epsilon, IMD18,CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
    分子量
    36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
    途径
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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