FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
蛋白类型
Recombinant
产品特性
AA 36-218
宿主
人
资源
HEK-293 Cells
标记
This FCGR2A protein is labelled with His-Avi Tag.
原理
Human Fc gamma RIIA/CD32a (H167) Protein
序列
Ala36-Ile218 (H167)
产品特性
Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala36-Ile218(H167).
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
The affinity constant of 0.606 μM as determined in SPR assay (Biacore T200). See testing image for detail.
FCGR2A
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein
背景
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
分子量
23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.