CD40 Protein (CD40) (AA 21-193) (His tag)
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- 抗原 See all CD40 蛋白
- CD40
- 蛋白类型
- Recombinant
- 产品特性
- AA 21-193
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD40 protein is labelled with His tag.
- 原理
- Human CD40/TNFRSF5 Protein
- 序列
- Glu21-Arg193
- 产品特性
- Recombinant Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Arg193.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD40, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 0.13μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CD40 蛋白
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD40
- 别名
- CD40 (CD40 产品)
- 别名
- Bp50 Protein, CDW40 Protein, TNFRSF5 Protein, p50 Protein, AI326936 Protein, GP39 Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TRAP Protein, Tnfrsf5 Protein, TNFSF5 Protein, CD40 molecule Protein, CD40 antigen Protein, CD40 Protein, Cd40 Protein
- 背景
- CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 Molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
- 分子量
- 20 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
- 途径
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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